Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers

Oxid Med Cell Longev . 2019 Jun 10;2019:5164576. doi: 10.1155/2019/5164576. eCollection 2019.

Fecha de la publicación: 10/06/2019

Autor: Miguel A Ortega (1), Beatriz Romero (1), Ángel Asúnsolo (2), Mayte Sola (1), María J Álavrez-Rocha (1), Felipe Sainz (3), Melchor Álavrez-Mon (4), Julia Buján (1), Natalio García-Honduvilla (1)



1Department of Medicine and Medical Specialties, Faculty of Medicine and Health Sciences, University of Alcalá, and Networking Biomedical Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain.

2Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcala, and Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain.

3Angiology and Vascular Surgery Service, Central University Hospital of Defense-UAH, Madrid, Spain.

4Immune System Diseases-Rheumatology and Oncology Service, University Hospital Príncipe de Asturias, Alcalá de Henares, Madrid, Spain.


Chronic venous insufficiency (CVI) is a disease that impacts cellular homeostasis. CVI may occur with a valvular destruction process known as venous reflux or valvular incompetence. One of the cellular processes that may be triggered as a consequence of these events is the production of reactive oxygen species (ROS), which may trigger the production of different cellular markers and cell damage processes, such as lipid peroxidation. Therefore, the present study performed an observational, analytical, and prospective cohort study by reviewing 110 patients with CVI, and the activities and plasma levels of iNOS, eNOS, NOX1, and NOX2 were determined using immunohistochemistry and RT-qPCR. Lipid peroxidation (MDA) was also measured. Patients were distributed according to the presence or absence of valvular incompetence-venous reflux, which was diagnosed clinically as the absence of venous reflux (NR = 29) or presence of venous reflux (R = 81). Each group was divided according to age, with a cutoff point of fifty years (NR < 50 = 13, NR ≥ 50 = 16, R < 50 = 32, and R ≥ 50 = 49). The results showed that R patients exhibited significantly increased plasma MDA levels, and R < 50 patients exhibited the highest statistically significant increase. iNOS, NOX1, and NOX2 exhibited the highest gene and protein expression in R patients. The increased expression was maintained in the R < 50 patients. Our data suggest that young patients with valvular incompetence (venous reflux) show higher levels of lipid peroxidation and oxidative stress, which reflects the characteristics of an aged patient.